The methods reviewed are: (i) the Sanger method and its most important variants (enzymic methods) (ii) the Maxam & Gilbert method and other chemical methods (iii) the Pyrosequencing TM method – DNA sequencing in real time by the detection of released pyrophosphate (PPi) and (iv) single molecule sequencing with exonuclease (exonuclease digestion of a single molecule composed of a single strand of fluorescently labelled deoxynucleotides). Important practical issues covered are read-length, speed, accuracy, throughput, cost, as well as the automation of sample handling and preparation. The four best known DNA sequencing techniques are reviewed.
Single molecule sequencing with exonuclease 190 Pyrosequencing – DNA sequencing in real time by the detection of released PPi 187Ħ. Maxam & Gilbert and other chemical methods 183ĥ. Sanger's method and other enzymic methods 170ģ.6.2 Mass spectrometry – an alternative 182Ĥ.
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